Additional file 1 of Extracellular vesicles derived from umbilical cord mesenchymal stromal cells alleviate pulmonary fibrosis by means of transforming growth factor-β signaling inhibition

Additional file 1: Figure S1. Effect of different concentrations of TGF-β1 on α-SMA expression. WML2 fibroblast cells were cultured for 48 h with different concentrations of TGF-β1 (0, 0.25, 5, and 10 ng/mL) to induce myofibroblast differentiation, and then the expression levels of α-SMA were detected in different treatments using IF (a) and qRT-PCR (b). Scale bar = 200 μm. Mean ± SEM; ***p < 0.001; n = 3. TGF-β1, transforming growth factor-β1; α-SMA, α-smooth muscle actin; Fn, fibronectin. Figure S2. uMSC lineage tracing in the lung tissue of BLM-induced mouse. uMSCs were labeled with PKH67 and then injected via mouse tail veins of BLM-induced mouse. uMSC tracing in the lung tissue was performed on days 1, 4, 7, and 14 after cell injection. Figure S3. uMSC-EVs lineage tracing in the lung tissue of BLM-induced mouse. uMSC-EVs were labeled with PKH67 and then injected via mouse tail veins of BLM-induced mouse. uMSC-EVs-tracing in the lung tissue were performed on days 1, 4, 7, and 14 after cell injection. Figure S4. Effects of uMSC-EVs on the expression levels of TGF-β1, TGF-β3 and TGF-βR1 in lung tissues of PF mice. a Expression levels of TGF-β1. b Expression levels of TGF-β3. c Expression levels of TGF-βR1. Scale bar = 200 μm. Table S1. Antibodies used in the surface antigen expression detection of uMSCs. Table S2. Primers.