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Additional file 1 of Efficient conversion of phytosterols into 4-androstene-3,17-dione and its C1,2-dehydrogenized and 9α-hydroxylated derivatives by engineered Mycobacteria

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posted on 2021-08-17, 11:03 authored by Xin Li, Tian Chen, Fei Peng, Shikui Song, Jingpeng Yu, Douanla Njimeli Sidoine, Xiyao Cheng, Yongqi Huang, Yijun He, Zhengding Su
Additional file 1. Supplementary informations include homologous recombinant sequences for knocking out targeted genes in the HGMS2 mutants, primers used for the construction of knockout and knockin vectors, antibiotic resistances of the HGMS2 strain, DNA sequence of KstD2 gene, DNA sequence of KshA51 gene, amino acid alignment of KstD211 with other KstD enzymes, amino acid alignment of KshA51 with the Ksh enzymes from the HGMS2 strain, and experimental data for PCR screening of KO and KI mutants.

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Hubei Technological Innovation Special Fund Department of Science and Technology, Hubei Provincial People's Government

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    Microbial Cell Factories

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    Keywords

    1,4-Androstadiene-3,17-dione (ADD)21-Hydroxy-20-methylpregn-4-en-3-one (BA)3-Ketosteroid-1,2-dehydrogenase (KstD)3-Ketosteroid-9α-hydroxylase (Ksh)4-Androstene-3,17-dione (4-AD)9α-Hydroxyl-4-androstene-3,17-dione (9OH-AD)Mycobacterium sp. strain

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