posted on 2024-01-04, 04:40authored byHae Jun Kwon, Joungmin Lee, Soo Jae Kwon, Hyun Sook Lee
Additional file 1: Supplementary Methods; Fig. S1. Transformation of unmethylated pKLJM082 and pKLJM083; Fig. S2. Schematic diagram of pyrE deletion in AWRP and confirmation of the genotype and phenotype; Table S1. Strains and plasmids used in this study; Table S2. Primers used in this study; Table S3. Guide RNA sequences for genome editing; Sequence of the synthesized guide RNA cassette for AsCas12a (cloned in pUC57)