Additional file 1 of Chronic high-sugar diet in adulthood protects Caenorhabditis elegans from 6-OHDA-induced dopaminergic neurodegeneration

Additional file 1: Fig. S1. Survival of worms during the 5-day exposure to high sugar. BY200 worms were reared to Day 1, at which point 20 worms per treatment per replicate were transferred to control NGM plates or plates containing 100 mM glucose or 100 mM fructose. Each day worms were assessed for survival by touch response with a flame sterilized platinum pick. Surviving worms were transferred to freshly seeded plates each day to avoid plate acidification and to separate them from progeny. For each group 3 biological replicates were assessed, with 20 individuals per replicate. A two-way ANOVA was performed to assess statistical significance, with no differences identified.