posted on 2024-03-13, 07:00authored byFrancesca Benedetti, Emmanuel F. Mongodin, Jonathan H. Badger, Arshi Munawwar, Ashley Cellini, Weirong Yuan, Giovannino Silvestri, Carl N. Kraus, Simone Marini, Chozha V. Rathinam, Marco Salemi, Hervé Tettelin, Robert C. Gallo, Davide Zella
Additional file 1: Figure S1. A Direct binding of eM-DnaK to ARV-1502 as determined by surface plasmon resonance (SPR). Association of ARV-1502 at different concentrations on 2274.9 response units of eM-DnaK immobilized on a CM5 biosensor chip proceeded at a flow rate of 35 μL/min for 250 s, followed by a 600 s dissociation in HBS-EP. A preliminary kinetic analysis yielded a Kd value of 1.899e−6M. B ARV-1502 binds to eM-DnaK and does not prevent eM-DnaK entry into HCT116 cells. eM-DnaK was incubated for 3 h with ARV-1502 and then added to HCT116 cells. After 24 h of incubation cells were treated for 48 h with cisplatin. Western blotting analysis shows that eM-DnaK is able to enter into HCT116 cell line despite the binding of ARV-1502 to DnaK and the treatment with cisplatin. Cells not treated with eM-DnaK, ARV-1502 and cisplatin were used as control.