Additional file 1 of Automated radiosynthesis of two 18F-labeled tracers containing 3-fluoro-2-hydroxypropyl moiety, [18F]FMISO and [18F]PM-PBB3, via [18F]epifluorohydrin

Additional file 1: Figure S1. The system diagram of automated multi-purpose synthesizer developed in-house (Fukumura et al. 2007). Figure S2. The preparative HPLC chromatogram of [18F]FMISO synthesized by 18F-fluorination using 1 and [18F]F-, followed by the removal of the protecting group in [18F]3. The HPLC conditions were as follows: XBridge C18 column (5 μm, 10 mm i.d. × 250 mm length; Waters), with a mixture of ethanol and water (2:98, vol./vol.) as the mobile phase, a flow rate of 5.0 mL/min, and UV detection at 325 nm. Figure S3. The preparative HPLC chromatograms of [18F]PM-PBB3 synthesized by 18F-fluorination using 2 and [18F]F-, followed by the removal of the protecting group in [18F]4. The HPLC conditions were as follows: Capcell Pak C18 column (5 μm, 10 mm i.d. × 250 mm length; Shiseido, Tokyo, Japan), the mixture of acetonitrile, water and triethylamine (40:60:0.1, v/v/v) as the mobile phase, 5.0 mL/min flow rate, and UV detection at 365 nm.