Additional file 1 of Aging gene signature of memory CD8+ T cells is associated with neurocognitive functioning in Alzheimer’s disease

Additional file 1: Supplementary Methods. 1.1. Gene Discovery. 1.2. RNA Isolation and Complementary DNA synthesis Protocol. 1.3. Quantitative Polymerase Chain Reaction. 1.4. Data Processing. 1.5. Statistical Analyses. Table S1. Gene Targets’ Primer Sequences. Table S2. Gene Targets Analyzed. Table S3. GLM analysis for differentially expressed genes. Table S4. LSM differences between clinical groups generated from GLM analysis for differentially expressed genes. Table S5. Molecular and biological pathways over-represented by differentially expressed genes. Figure S1.1. Top IL-7Rαlow aging genes. Figure S1.2. IL-7Rαlow aging genes downregulated in at least 2 AD datasets and were not associated with Memory Dataset. Figure S1.3. IL-7Rαlow aging genes associated with AD per MsigDB and upregulated in at least 2 AD datasets. Figure S1.4. IL-7Rαlow aging genes associated with Memory Dataset and AD per MsigDB and/or upregulated in at least 2 AD Datasets. Figure S1.5. AD genes associated with memory database, not IL-7Rαlow aging genes. Figure S1.6. Genes and downregulated in 3 AD datasets; not IL-7Rαlow aging genes. Figure S1.7. Inflammatory control genes. Figure S2. All Genes PCA Plot. Figure S3. Hierarchal Clustering Heatmap. Figure S4. MoCA and CDRsob Scores per Dementia Cluster. Figure S5.1. Processing Speed Z-scores vs. Gene Expression Z-scores. Figure S5.2. Verbal Memory Z-scores vs. Gene Expression Z-scores. Figure S5.3. Episodic Memory Z-scores vs. Gene Expression Z-scores. Figure S5.4. Executive Function Z-scores vs. Gene Expression Z-scores. Figure S5.5. Language Z-scores vs. Gene Expression Z-scores. Figure S5.6. Visuospatial Ability Z-scores vs. Gene Expression Z-scores.