posted on 2022-02-06, 04:24authored byDan Sun, Liuhong Zhu, Le Guo, Shaoli Wang, Qingjun Wu, Neil Crickmore, Xuguo Zhou, Alejandra Bravo, Mario Soberón, Zhaojiang Guo, Youjun Zhang
Additional file 1: Figure S1. Diagram of the crossing strategy to obtain homozygous double knockout strains in P. xylostella. Figure S2. Diagram of the crossing strategy used for generation of the genetic hybrid strain. Table S1. Primers used in this study. Table S2. Toxicity to Cry1Ac toxin in larvae from the polygenic knockout strains and their F1 progeny.
Funding
Laboratory of Lingnan Modern Agriculture Project National Natural Science Foundation of China Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences