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Additional file 1: Figure S1. of Changes in peripheral immune cell numbers and functions in octogenarian walkers – an acute exercise study

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journal contribution
posted on 2017-02-22, 05:00 authored by Kornelis van der Geest, Qi Wang, Thijs Eijsvogels, Hans Koenen, Irma Joosten, Elisabeth Brouwer, Maria Hopman, Joannes Jacobs, Annemieke Boots
Exercise-induced T cell proliferation rates and T cell subset redistribution in CMV+ and CMV- subjects. (a) Rates of CD4 (left panel) and CD8 (right panel) proliferation before (Pre-Walking) and after exercise (Post-Walking) assessed by Ki-67 expression using flow-cytometry. Percentages of Ki-67 expressing cells within the CD4 and CD8 populations are shown. (b) Enumeration of CD4 and CD8 T cell differentiation subsets based on CD45RO and CCR7 expression Pre- and Post-Walking in CMV seropositive subjects (upper panel, n = 13) and CMV seronegative subjects (lower panel, n = 7). Mean (+/- SEM) numbers (109 cells/L) of naïve T cells (TNaïve), central memory T cells (TCM), effector memory T cells (TEM) and terminally differentiated T cells (TTD) are shown. (c) Mean (+/- SEM) numbers (109 cells/L) of recent thymic emigrants defined as CD31 + CD4 + TNaïve and the central naïve CD31-CD4+ TNaïve subsets in CMV+ and CMV- subjects. Statistical significance by Wilcoxon signed rank test is indicated as *p < 0.05, **p < 0.01. (DOCX 995 kb)


Koninklijke Nederlandse Akademie van Wetenschappen