Additional analysis of initial axon morphology.

Light-sheet microscopy of cleared rat DRG; axon lengths (A), stem diameter (B), and somatic diameter (C) of the peripherin- and NF-200-labeled fibers were analyzed per animal (see also Fig 6C–6E). (A) Peripherin: 3 animals (rat 1: 5 DRGs, 36 stems; rat 2: 2 DRGs, 9 stems; rat 3: 3 DRGs, 12 stems). NF200: 3 animals (rat 1: 4 DRGs, 22 stems; rat 2: 1 DRG, 3 stems; rat 3: 2 DRGs, 5 stems); paired t test: t(2) = 3, p < 0.05. (B) Peripherin: 3 animals (rat 1: 5 DRGs, 36 stems; rat 2: 2 DRGs, 9 stems; rat 3: 3 DRGs, 12 stems). NF200: 3 animals (rat 1: 4 DRGs, 22 stems; rat 2: 1 DRG, 3 stems; rat 3: 2 DRGs, 5 stems); paired t test: t(2) = 3, p < 0.05. (C) Peripherin: 3 animals (rat 1: 5 DRGs, 150 somas; rat 2: 2 DRGs, 122 somas; rat 3: 3 DRGs, 155 somas). NF200: 3 animals (rat 1: 4 DRGs, 150 somas; rat 2: 1 DRG, 31 somas; rat 3: 2 DRGs, 75 somas); paired t test: t(2) = 7, p < 0.05. Metadata for quantifications presented in this figure can be found at https://archive.researchdata.leeds.ac.uk/1042/.

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