posted on 2024-02-26, 18:42authored byMagdalena Murr, Conrad Freuling, David Pérez-Bravo, Christian Grund, Thomas C. Mettenleiter, Angela Römer-Oberdörfer, Thomas Müller, Stefan Finke
Chicken embryo fibroblasts (DF-1) (A), bovine kidney cells (MDBK) and baby hamster kidney cells (BHK-21) (B) were infected with rNDV or rNDV_GRABV (multiplicity of infection (moi) 5) and harvested 24 h post infection (p. i.). Cell lysates and lysates of purified virions (10 μg per lane) (C) were subjected to SDS-PAGE and subsequently to Western blot analysis. Viral proteins were visualized by immunostaining with respective antibodies. Β-Actin was detected on every cell lysate blot as loading control. Blots of cell lysates are representative of three biological replicates. Blot of purified virions were done once. The figure depicts the uncompartmentalized illustrations of the western blots belonging to Fig 3 of the manuscript.