posted on 2021-07-28, 23:44authored byHui Zhang, Chang Liu, Wenyi Hua, Lucien P. Ghislain, Jianhua Liu, Lisa Aschenbrenner, Stephen Noell, Kenneth J. Dirico, Lorraine F. Lanyon, Claire M. Steppan, Mike West, Don W. Arnold, Thomas R. Covey, Sammy S. Datwani, Matthew D. Troutman
We describe a mass
spectrometry (MS) analytical platform resulting
from the novel integration of acoustic droplet ejection (ADE) technology,
an open-port interface (OPI), and electrospray ionization (ESI)-MS
that creates a transformative system enabling high-speed sampling
and label-free analysis. The ADE technology delivers nanoliter droplets
in a touchless manner with high speed, precision, and accuracy. Subsequent
sample dilution within the OPI, in concert with the capabilities of
modern ESI-MS, eliminates the laborious sample preparation and method
development required in current approaches. This platform is applied
to a variety of experiments, including high-throughput (HT) pharmacology
screening, label-free in situ enzyme kinetics, in vitro absorption,
distribution, metabolism, elimination, pharmacokinetic and biomarker
analysis, and HT parallel medicinal chemistry.