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A subnanosecond pulsed electric field system for studying cells electropermeabilization
journal contribution
posted on 2021-01-05, 11:59 authored by N Ibrahimi, L Vallet, FM Andre, L Ariztia, M Rivaletto, AS De Ferron, Bucur NovacBucur Novac, LM Mir, L Pécastaing© 2020 IEEE. This article presents an experimental arrangement which, using 3-D numerical modeling, aims to study biomedical effects using subnanosecond pulsed electric fields (PEFs). As part of a major effort into developing contactless technology, the final aim of this study is to determine conditions of the applied PEFs (number of pulses, strength, pulse repetition frequency) able to produce electropermeabilization. The arrangement uses a pulsed power generator producing voltage impulses with an amplitude of up to 20 kV on a 50- Ω matched load, with a rise time of 100 ps and a duration of 600 ps. During the preliminary study reported here, samples containing E. Coli were exposed to PEFs in a 4-mm standard electroporation cuvette, allowing the application of a peak electric field strength of up to 60 kV/cm. The studies were facilitated by detailed 3-D electromagnetic modeling of the electric field distribution generated by voltage impulses inside the system. Due to the nature of tests, the numerical analysis played an essential role in the interpretation of results. Preliminary biological results reported in this study are very encouraging, showing that trains of 5000 to 50 000 pulses applied at a pulsed repetition frequency of 200 Hz (maximum PRF) can efficiently induce E. Coli electropermeabilization.
Funding
Agence Nationale de la Recherche (ANR), under Contract No. ANR-18- ASTR-0019
History
School
- Mechanical, Electrical and Manufacturing Engineering
Published in
IEEE Transactions on Plasma ScienceVolume
48Issue
12Pages
4242 - 4249Publisher
IEEEVersion
- AM (Accepted Manuscript)
Rights holder
© IEEEPublisher statement
Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.Acceptance date
2020-10-24Publication date
2020-11-10Copyright date
2020ISSN
0093-3813eISSN
1939-9375Publisher version
Language
- en
Depositor
Prof Bucur Novac. Deposit date: 2 January 2021Usage metrics
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No categories selectedKeywords
Biological cellselectropermeabilizationpulsed electric fields (PEFs)pulsed powersubnanosecond pulsesScience & TechnologyPhysical SciencesPhysics, Fluids & PlasmasPhysicsINDUCE APOPTOSISELECTROPORATIONSTIMULATIONPERFORMANCEDELIVERYVOLTAGEANTENNADESIGNFluids & PlasmasAtomic, Molecular, Nuclear, Particle and Plasma PhysicsElectrical and Electronic Engineering
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