posted on 2021-07-21, 18:08authored byJie Yuan, Tian-Bing Ren, Shuai Xu, Chun-Jiang Wang, Xiao-Bing Zhang, Lin Yuan
With
the increasing interest in photodynamic therapy (PDT), the
assessment of the level of reactive oxygen species produced during
PDT has also become increasingly important. However, most of the fluorescent
probes for reactive oxygen species (ROS) evaluation were separated
from photosensitizers in the PDT process, resulting in ex
situ and asynchronous treatment feedback. Additionally, the
consumption of ROS by these fluorescent probes themselves will inevitably
affect the therapeutic effect. Herein, inspired by the redox balance
in the cell, we developed a multifunctional hydrogen sulfide (H2S) probe Ru-NBD for reporting the therapeutic effect during
the PDT process by detecting hydrogen sulfide. The probe Ru-NBD could
not only serve as an effective PDT reagent both before and after H2S activation but could also be used for real-time and in situ monitoring of the therapeutic effect via restored luminescence during the PDT process. As the phototherapy
process progresses, the luminescent signal of Ru-NBD changes accordingly.
The experimental results show that there is a certain correlation
between the luminescence intensity and the cell inhibition rate; thus,
we can monitor the phototherapy process by detecting the changes in
the probe’s luminescent signal. This study provides an idea
for the design and adjustment of PDT.