posted on 2024-03-18, 14:41authored byYunhua Guo, Wenzhe Song, Yuhang Dong, Xuejun Wang, Guangjun Nie, Feng Li
Broad-spectrum antiviral platforms are always desired
but still
lack the ability to cope with the threats to global public health.
Herein, we develop a poly aptamer encoded DNA nanocatcher platform
that can trap entire virus particles to inhibit infection with a broad
antiviral spectrum. Ultralong single-stranded DNA (ssDNA) containing
repeated aptamers was synthesized as the scaffold of a nanocatcher
via a biocatalytic process, wherein mineralization of magnesium pyrophosphate
on the ssDNA could occur and consequently lead to the formation of
nanocatcher with interfacial nanocaves decorated with virus-binding
aptamers. Once the viruses were recognized by the apatmers, they would
be captured and trapped in the nanocaves via multisite synergistic
interactions. Meanwhile, the size of nanocatchers was optimized to
prevent their cellular uptake, which further guaranteed inhibition
of virus infection. By taking SARS-CoV-2 variants as a model target,
we demonstrated the broad virus-trapping capability of a DNA nanocatcher
in engulfing the variants and blocking the infection to host cells.