posted on 2023-11-07, 13:11authored byZhicheng Yan, Yiyao Pan, Guofeng Jiao, Mengyu Xu, Dongguang Fan, Ziwei Hu, Jiarui Wu, Tao Chen, Miao Liu, Xiaoguang Bao, Hengte Ke, Xingyue Ji
Bioorthogonal
decaging chemistry with both fast kinetics and high
efficiency is highly demanded for in vivo applications but remains
very sporadic. Herein, we describe a new bioorthogonal decaging chemistry
between N-oxide and silylborane. A simple replacement
of “C” in boronic acid with “Si” was able
to substantially accelerate the N-oxide decaging
kinetics by 106 fold (k2: up
to 103 M–1 s–1). Moreover,
a new N-oxide-masked self-immolative spacer was developed
for the traceless release of various payloads upon clicking with silylborane
with fast kinetics and high efficiency (>90%). Impressively, one
such N-oxide-based self-assembled bioorthogonal nano-prodrug
in combination with silylborane led to significantly enhanced tumor
suppression effects as compared to the parent drug in a 4T1 mouse
breast tumor model. In aggregate, this new bioorthogonal click-and-release
chemistry is featured with fast kinetics and high efficiency and is
perceived to find widespread applications in chemical biology and
drug delivery.