art%3A10.1007%2Fs00441-016-2430-x.pdf (1.89 MB)
Function of extracellular vesicle-associated miRNAs in metastasis
journal contribution
posted on 2018-09-10, 12:47 authored by Bert DhondtBert Dhondt, Quentin Rousseau, Olivier De Wever, An HendrixExtracellular RNA (exRNA) is functionally transferrable from donor to recipient cells and is protected from RNAses by electrostatic interactions with proteins or by membrane encapsulation. In addition to bioactive RNA, extracellular vesicles (EVs) contain intraluminal and membrane-associated proteins. The cellular context and fitness affect the composition of EVs and thus the outcome of the communication between the EV-producer and recipient cells. Adaptive communication through EVs is particularly important between cancer cells and their local and distant environment and drives life-threatening metastatic progression. Small noncoding RNAs (miRNAs) have been reported in EV isolations and play a role in local invasion, angiogenesis, immune modulation, metastatic niche preparation, colonization and dormancy. The metastasis-related functions attributed to EV-associated miRNAs are currently increasing exponentially in the scientific literature. We must be aware that the correct and efficient separation of non-vesicular entities (soluble proteins, RNA-protein complexes and RNA-lipoprotein complexes) from EVs is necessary to determine the true contribution of EVs in any experiment that describes the molecular content or the functional consequences of the isolated material.
History
Usage metrics
Categories
- Cancer cell biology
- Cancer genetics
- Cell physiology
- Solid tumours
- Tumour immunology
- Medical biochemistry and metabolomics not elsewhere classified
- Biochemistry and cell biology not elsewhere classified
- Oncology and carcinogenesis not elsewhere classified
- Genomics
- Other biomedical and clinical sciences not elsewhere classified
- Plant cell and molecular biology
- Animal cell and molecular biology
- Protein trafficking
- Signal transduction
Keywords
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC