Efficient Way to Generate Protein-Based Nanoparticles by in-Situ Photoinitiated Polymerization-Induced Self-Assembly

Protein-based nanoparticles with tailored properties by using different functional proteins as building blocks have many actual and potential applications in biomedicine, biotechnology, and nanotechnology. In this study, we demonstrated a facile and efficient way to synthesize protein-based nanoparticles by taking advantage of photoinitiated reversible addition–fragmentation chain transfer (RAFT) polymerization-induced self-assembly by using multi-RAFT modified bovine serum albumin (BSA) as a macro-RAFT agent. The growth of the PHPMA chains results in the increase of the hydrophobicity of the star BSA–PHPMA conjugates, and when reaching the critical aggregation concentration in aqueous solution, they will aggregate into nanoparticles via the hydrophobic interaction of PHPMA. The generated nanoparticles also showed excellent encapsulation ability toward both hydrophobic and hydrophilic components, and as a proof of concept, after loading cancer drug DOX or biomacromolecule DNA, the protease-mediated release of the encapsulants was demonstrated. It is anticipated that the described method may open up new opportunities for designing a variety of protein–polymer self-assembled nanostructures tailored to specific applications.