pone.0297623.s001.docx (130.23 kB)
48 samples of viral RNA isolated from 24 honey bee pooled samples.
journal contribution
posted on 2024-03-14, 17:32 authored by Sofia Levin Nikulin, Poppy J. Hesketh-Best, Dean A. Mckeown, Marla Spivak, Declan C. SchroederQueen label represents a queen line. Viral load expressed as log10 viral RNA copies/ a pooled sample. Total sample volume was 19 ml; Sample input volume was 200ul (50ul of filtrate + 150ul of 1xPBS) and 150ul (50ul of filtrate + 100ul of 1xPBS) for magnetic beads and columns affinity extraction methods respectively.
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magnetic bead captureleast 300 yearshoney bee viruseshoney bee coloniesdead honey beesbased purification methodmonitor viral loadsautomated method describedhoney beesviral loadsscreening colonieseffective methodongoing declinefirst detectedfaster screeningeasily appliedbeekeeping industryautomated approachanalytical results96 samples
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