ac5b04278_si_001.pdf (685.65 kB)
Direct Biofluid Analysis Using Hydrophobic Paper Spray Mass Spectrometry
journal contribution
posted on 2016-02-10, 18:30 authored by Deidre
E. Damon, Kathryn M. Davis, Camila R. Moreira, Patricia Capone, Riley Cruttenden, Abraham K. Badu-TawiahAmbient electrostatic paper spray
ionization from a hydrophobic
paper occurs when a DC potential is applied to the dry paper triangle.
Online liquid/liquid extraction of small organic compounds from a
drop of biological fluid present on the dry hydrophobic paper is achieved
with an organic spray solvent in under 1 min and utilizes in situ
electrostatic-spray ionization for more efficient detection of extracted
molecules. Direct analysis of small volumes of biofluids with no sample
pretreatment is possible, which is applicable in point-of-care analyses.
High sensitivity and quantitative accuracy was achieved for the direct
analysis of illicit drugs in 4 μL of raw blood, serum, and whole
urine. The study was extended to monitor the activity of alanine transaminase
enzyme, a key biomarker for the detection of liver injury in patients
(with HIV and tuberculosis) who typically take several medications
at once.