siRNA-mediated StarD7 silencing impairs MTOC reorientation and disrupts the Golgi apparatus.

(A) The monolayer of siC or siD7 cells was wounded and 6 hours later cells were stained with anti-γ-tubulin (red) to detect MTOC (arrows). The boxed regions are enlarged in the right panels. The nuclei were labeled with Hoechst (blue), merged images are shown on the left. The images were recorded by fluorescence microscopy and white lines indicate the wound edge. Scale bar = 10 μm (600x). Bar graph shows the percentage of siC and siD7 cells exhibiting oriented MTOC after wound from two independent experiments (mean ± SEM, *p< 0.05, Student’s t-test). (B, C) Golgi apparatus morphology was visualized by fluorescence microscopy in siC and siD7 cells using anti-GM130 or anti-Golgin 97 antibodies, respectively (red). The nuclei were labeled with Hoechst (blue). Scale bar = 30 μm (200x). The boxed regions are enlarged in the right panels. Bar graph shows the percentage of siC and siD7 cells with fragmented Golgi from two independent experiments (mean ± SEM, *p< 0.05, Student’s t-test).

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