miR 212-5p expression is increased in aortic tissues from angiotensin II-treated mice and VSMCs.

(A) Systolic blood pressure was measured in vehicle- and angiotensin II-treated mice (n = 12 per group). (B) Representative images of H&E staining of aortic tissues (n = 6 per group). Scale bar = 100 μm. (C) Quantified aortic wall thickness (n = 6 per group) in vehicle- and angiotensin II-treated mice. (D) Aortic miR-212-5plevels were evaluated using RT-PCR. ***P < 0.001 versus the vehicle-treated group. (E) VSMCs were serum-starved and then treated with angiotensin II (1 μM) for 1 day. miR-212-5p levels were evaluated by RT-PCR. **P < 0.01 versus the vehicle-treated group. (F,G) Aortic miR-212-3p and miR-6937-5p levels were evaluated using RT-PCR. NS indicates not significant.