miR-212-5p overexpression attenuates VSMC proliferation and migration.

(A) VSMCs were transfected with control or miR-212-5p mimic for 2 days. Cell proliferation was determined by the MTT assay. **P < 0.01 versus the control mimic group. (B) Direct cell counting showing in vitro proliferation of VSMCs transfected with miR-212-5p mimic. **P < 0.01 versus the control mimic group. (C) After transfection of VSMCs with miR-212-5p mimic, cyclin D1 transcript levels were determined by RT-PCR. *P < 0.05 versus the control mimic group. (D,E) VSMCs transfected with miR-212-5p mimic were incubated with BrdU and then immunostained with an anti-BrdU antibody. Scale bar = 100 μm. The number of BrdU-positive cells was quantified. **P < 0.01 versus the control mimic group. (F,G) VSMCs were transfected with control or miR-212-5p mimic, and cell migration was assessed for 15~24 h by the scratch-wound assay. **P < 0.01 versus the control mimic group.