miR-212-5p inhibition increases VSMC proliferation and migration.

VSMCs were transfected with control or miR-212-5p inhibitor for 2 days. (A) Cell proliferation was determined by the MTT assay. **P < 0.01 versus the control inhibitor group. (B) After transfection of VSMCs with miR-212-5p inhibitor, cyclin D1 transcript levels were determined by RT-PCR. ***P < 0.001 versus the control inhibitor group. (C,D) VSMCs transfected with miR-212-5p inhibitor were incubated in the presence of BrdU and then immunostained with an anti-BrdU antibody. Scale bar = 100 μm. The number of BrdU-positive cells was quantified. ***P < 0.001 versus the control inhibitor group. (E,F) VSMCs were transfected with control or miR-212-5p inhibitor, and cell migration was assessed for 15~24 h by the scratch-wound assay. **P < 0.01 versus the control inhibitor group.