mDia regulates HSPCs aging and colony formation under re-seeding stress.

(A) Peripheral blood cell counts of 2-year-aged control and mDia2 conditional KO mice (mDia2^fl/fl Vav-Cre). (B) Flow cytometric analyses of the percentage and total cell counts of indicated HSPC subpopulations in the bone marrow from mice in A. (C) Proportion of bone marrow LSK cells in each stage of the cell cycle (G0, G1, S-G2/M) from the indicated aged mice. (D-E) Quantification of CFU numbers as in D and colony size as in E with serial plating of bone marrow cells from indicated wild type or mDia1 KO mice performed in triplicate. 1°: 125 colonies in WT, and 133 colonies in mDia1 KO; 2°: 286 colonies in WT, and 225 colonies in mDia1 KO. Error bars represent the SEM of the mean. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Two-tailed unpaired student’s t-test was used to generate the p values.

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