figshare
Browse
pone.0260155.g002.tif (947.2 kB)

iNSA/E6E7equ and iNSB/E6E7equ were genetically stable and expressed shuffled E6/E7 protein.

Download (947.2 kB)
figure
posted on 2021-11-19, 18:32 authored by Christoph Jindra, Edmund K. Hainisch, Andrea Rümmele, Markus Wolschek, Thomas Muster, Sabine Brandt

Top: Transgene stability in the iNS1 reading frame was demonstrated by RT-PCR from total RNA after five passages of iNSA/E6E7equ and iNSB/E6E7equ in Vero cells. Bottom: Western blot analysis of lysates of equine primary palatal fibroblasts (eqPALF) infected with iNSA/E6E7equ (left) and iNSB/E6E7equ (right) revealed stable influenza virus transgene (E6E7) expression.

History

Usage metrics

    PLOS ONE

    Categories

    Keywords

    unsuccessful therapeutic attemptstumour burden eventruncated ns1 genetransient side effectsystemic antitumour responseplaque assay revealedmay 2021 ).former tumour sitesassuring interferon inductionaffected individuals duesarcoids seriously compromiseb virus vectorsequ div >< pcomplete tumour regressionvirus sheddingmultiple sarcoidswell toleratedvaccination schemessynchronous regressionstill ongoingsignificantly reducingsevere formscrapings collectedsarcoid immunotherapyrepeated screeningproved safemild feverintradermal administrationinduced singlee7 antigensdeveloped influenza

    Licence

    CC BY 4.0

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC