ppat.1011003.s002.tiff (361.5 kB)
(related to Figs 2, 3, 4, 5, 6 and 7).
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posted on 2023-01-05, 18:42 authored by Shishir Singh, Mateo Murillo-León, Niklas Sebastian Endres, Ailan Farid Arenas Soto, Jorge Enrique Gómez-Marín, Florence Melbert, Thirumala-Devi Kanneganti, Masahiro Yamamoto, Claudia Campos, Jonathan Charles Howard, Gregory Alan Taylor, Tobias SteinfeldtGeneration of T. gondii RHΔrop39 and RHΔrop18/rop39 deletion mutants. A, Schematic representation of specific gRNA-mediated targeting of CRISPR/Cas9 to the endogenous rop39 locus and integration of the DHFR selection cassette carrying rop39 5`and 3`UTR homology regions. B, Diagnostic PCR of gDNA prepared from clonal lines demonstrates excision and replacement of the rop39 exon with the DHFR selection cassette.
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parasitophorous vacuolar membraneinhibits homodimer formationdirect interaction partnerspvm integrity rescuespolymorphic rhoptry proteinszoonotic apicomplexan parasiteirg resistance systemtoxoplasma gondii specific parasite effectorirg proteinsspecific inactivationpvm ).gondii irg proteinyet unknownvivo vitro study identifiessmall rodentsrelated gtpasesoverall reductionone thirdmediated clearanceinducible immunityhuman populationgtpase leadingglobal scaledomestic catdefinitive hostdata revealclinical disability
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