Figure_2.tif (5.07 MB)

p-STAT5^Tyr694 and p-STAT3^Tyr705 expression upon GH treatment compared to control mice.

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posted on 2014-07-11, 02:54 authored by Michaela Schlederer, Kristina M. Mueller, Johannes Haybaeck, Susanne Heider, Nicole Huttary, Margit Rosner, Markus Hengstschläger, Richard Moriggl, Helmut Dolznig, Lukas Kenner

A Western Blot of p-STAT5^Tyr694 expression in Stat5ab^+/+ (n = 2), Stat5ab^+/Δ (n = 2) and Stat5ab^Δ/Δ (n = 2) livers of GH treated and control mice. HSC70 is shown as loading control. B IHC of p-STAT5^Tyr694 in sections of the livers (n = 3) used in A. Red, AEC; blue, hematoxylin. Size bar: 100 µm. High magnifications (60x) are shown in insets. C Quantification of p-STAT5^Tyr694 levels from Western blots (grey) by densitometric scanning and IHC (magenta) using the HistoQuest software (Stat5ab^+/+, control; number of cells n = 3276, GH; number of cells n = 2286; Stat5ab^+/Δ control; number of cells n = 2577, GH; number of cells n = 3549; Stat5ab^Δ/Δ control; number of cells n = 3299, GH; number of cells n = 2200). The highest arbitrary expression values from A and B were set to 100 for direct comparison. D Western blot of p-STAT3^Tyr705 expression in Stat5ab^+/+ (n = 2), Stat5ab^+/Δ (n = 2) and Stat5ab^Δ/Δ (n = 2) livers of GH treated and control mice. HSC70 is shown as loading control. E IHC of p-STAT3 ^Tyr705 in sections of the livers (n = 3) used in D. Red, AEC; blue, hematoxylin. Size bar: 100 µm. High magnifications are shown in insets (60x). F Quantification of p-STAT3^Tyr705 levels from Western blots (grey) by densitometric scanning and IHC (magenta) using the HistoQuest software (Stat5ab^+/+, control; number of cells n = 2634, GH; number of cells n = 2286; Stat5ab^+/Δ control; number of cells n = 22230, GH n = 2562; Stat5ab^Δ/Δ control; number of cells n = 2414, GH n = 2716). The highest arbitrary expression values from A and B were set to 100 for direct comparison.