pyrF expression has negligible impact on Δ trmB_Har fitness and transcriptome.

A: Fitted, log-transformed growth curves showing that AKS319 phenocopies AKS133, and (B) that there is no significant difference between AKS133 and AKS319 in 25 mM glucose as measured by the area under the curve (AUC). Strain colors are preserved in A and B. ** p-value < 0.01; **** < 0.0001. C: No significant differences in the growth rate of AKS319 cultures prior to RNA extraction between replicates exhibiting pyrF expression and not. Optical density measurements of the cultures harvested for RNA-seq are shown, with corresponding pyrF counts summarized in the table below. D: Average counts per transcript are highly correlated across AKS319 samples regardless of pyrF expression for both -glucose (N = 2) and +glucose conditions (N = 2). Average counts per transcript are highly correlated across AKS319 and AKS133 regardless of pyrF expression for both -glucose (N = 6) and +glucose conditions (N = 8). Average pyrF counts for each comparison are indicated in orange. Data are normalized relative to library size but have not been batch corrected.

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