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ΔtrmB growth.

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posted on 2024-01-16, 18:29 authored by Rylee K. Hackley, Angie Vreugdenhil-Hayslette, Cynthia L. Darnell, Amy K. Schmid

A: In-trans complementation of Δ trmBHar in both AKS133 and AKS319 backgrounds. Log-transformed, fitted growth curves of complementation strains and strains harboring the empty vector (EV) grown in the presence or absence of glucose. Shaded regions depict the 95% confidence intervals. B: Area under the growth curve (AUC) of (A), with FDR-corrected significance scores. C: Parent strain and Δ trmBHar growth in each condition relative to no carbon, measured by AUC. All growth experiments were done with a minimum of 3 biological replicates, each in technical triplicate. Error bars depict the standard deviation of the mean.

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