posted on 2024-01-16, 18:29authored byRylee K. Hackley, Angie Vreugdenhil-Hayslette, Cynthia L. Darnell, Amy K. Schmid
A: In-trans complementation of ΔtrmBHar in both AKS133 and AKS319 backgrounds. Log-transformed, fitted growth curves of complementation strains and strains harboring the empty vector (EV) grown in the presence or absence of glucose. Shaded regions depict the 95% confidence intervals. B: Area under the growth curve (AUC) of (A), with FDR-corrected significance scores. C: Parent strain and ΔtrmBHar growth in each condition relative to no carbon, measured by AUC. All growth experiments were done with a minimum of 3 biological replicates, each in technical triplicate. Error bars depict the standard deviation of the mean.