bfc knockout reduced crq expression without affecting macrophage development in the embryos.

A. The crq, drpr, and simu mRNA levels were quantified by qPCR in stage 13 wild-type, bfc^ko, and srp mutant embryos. B. Macrophages were stained with anti-CRQ (green) antibodies, and ACs were stained with anti-Dcp1 (magenta) antibodies in stage 13 wild-type and bfc^ko embryos. Scale bar = 20 μm. C. Graph showing the number of macrophages ± SEM for genotype of wild type, bfc^ko, srp-Gal4>UAS-GFP and bfc^ko; srp-Gal4>UAS-GFP. Statistical significance was assessed using the two-way ANOVA (A) or one-way ANOVA (C).

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