TRP1 prototrophy confers growth advantage in the presence of SDS.
figureposted on 2019-03-11, 17:29 authored by Lea Schroeder, Amy E. Ikui
(A) The indicated cells were 10-fold serially diluted onto YPD or SD plates with or without 0.0075% SDS. (B) Log phase cells of the genotype ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 (trp1-1) and isogenic TRP1 cells (TRP1) were grown in YPD with or without SDS (0.001%). Every hour for 4 hours the optical density (OD) was read and an aliquot of cells was stained with propidium iodide and analyzed by FACS. The bottom row is an example of the FACS readings at the 4 hour time-point and the gating used to determine live vs dead cells. The graph on the left indicates the percentage of dead cells. The graph on the right indicates the doubling times as the slope of the line determined by plotting log2 of the optical density reading vs. time.
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cell wall integritysodium dodecyl sulfatesodium dodecyl sulfate perturbationcell wall stressTRPMCK 1 genesodium dodecyl sulfate treatmentSDS-associated cell membrane stressactivates cell wall integritytea tree oiltrp 1-1 cellscell growthcell wall integrity pathwayTAT 2 tryptophan permease rescues cell growthsuppressor gene screeningSaccharomyces cerevisiae Sodium dodecyl sulfate