N. bombycis coated with Vg in the ovarioles of S. litura and H. armigera.

(A) Verification of polyclonal antibodies specifically recognizing the S. litura vitellogenin and H. armigera vitellogenin by western blotting. (B) Spores were isolated from female S. litura and H. armigera pupae, and labeled with Vg polyclonal antibody. (C) Colocalization of N. bombycis and Vg in S. litura ovarioles. (D) Colocalization of N. bombycis and Vg in H. armigera ovarioles. The arrowhead shows the parasite with a Vg signal; N. bombycis spores were stained using FB28 (blue); proliferative N. bombycis was labeled using rabbit anti-N. bombycis polyclonal antibody (Alexa488, green); S. litura Vg was detected using mouse anti-SlVg polyclonal antibody (Alexa647, yellow); H. armigera Vg was detected using mouse anti-HaVg polyclonal antibody (Alexa647, yellow); nuclei were stained using PI (red). OS, ovariole sheath; FC, follicular cell; O, oocyte; NC, nurse cell.

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