N. bombycis coated with BmVg in hemolymph and ovariole sheath.

(A, B) N. bombycis coated with BmVg in the ovariole sheath cells, the white dashed lines indicate the infected hemolymph cells. (C) N. bombycis coated with BmVg during infecting the oocyte from FCs, the white dashed lines indicate the boundaries of FCs. (D, E) N. bombycis coated with BmVg in the oocyte. The arrowhead shows N. bombycis spores. N. bombycis spores were stained with FB28 (blue); N. bombycis in proliferation was labelled by a rabbit anti-N. bombycis polyclonal antibody conjugated with Alexa Fluor 488 (green); the yellow fluorescence indicates the BmVg labeled by anti-BmVg conjugated with Alexa Fluor 647; nuclei were stained using PI (red). OS, ovariole sheath; FC, follicular cell; O, oocyte; NC, nurse cell.

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