LncR492 inhibits specifically neural differentiation.

(A) Gene expression analysis by qRT-PCR in Sox1-GFP ESCs during 4 days of differentiation in N2B27. Data presents the mean ± SD of three independent experiments.

(B) Immunofluorescent analysis of Sox1-GFP ESC after 72h of differentiation. Endogenous GFP (green), Tubb3 (red) and DAPI (blue) are shown. Scale bar 50 μm. Bar graph shows quantification of Tubb3 positive cells, which were normalized to the overall cell number.

(C) Time course analysis of gene expression by qRT-PCR after lncR492 knock-down in Sox1-GFP ESCs. Data presents mean ± SD of three independent experiments.

(D) Transient overexpression of lncR492 analysed by qRT-PCR in Sox1-GFP ESCs. Data presents the mean ± SD of three independent experiments. EV—empty vector.

(E) FACS analysis of % Sox1-GFP positive cells after overexpression of lncR492 and 4 days of differentiation. Data presents mean ± SD of 4 independent experiments.

(F) FACS analysis of % T-GFP positive cells after overexpression of lncR492 and 4 days of differentiation. Data presents mean ± SD of 4 independent experiments.

(G) FACS analysis of % Foxa2-GFP positive cells after overexpression of lncR492 and 4 days of differentiation. Data presents mean ± SD of 4 independent experiments.

* p<0.05; ** p<0.01; *** p<0.001; n.s.–not significant.