figshare
Browse
pone.0287865.g003.tif (5.75 MB)

Ldh expression in ganglion mother cells, neurons, and glia.

Download (5.75 MB)
figure
posted on 2024-01-03, 18:32 authored by Madhulika Rai, Sarah M. Carter, Shefali A. Shefali, Geetanjali Chawla, Jason M. Tennessen

Representative confocal images showing the absence of Ldh in neuroblasts of third instar Ldh-GFP genomic larvae. The first two columns from left to right show DAPI and Ldh. The last column shows the colocalization of Ldh with CNS cell specific markers. (A-D) Ldh is not expressed in neuroblasts, which are labelled with aMiranda (MIRA) antibody. In contrast, (E-H) Ldh is expressed in ganglion mother cells (GMCs), which are labelled with aProspero (PROS) antibody. Ldh-GFP is also present in both (I-L) neurons stained with aElav and (M-P) and glia stained with aRepo. The scale bar in all images represents 40 μM. Scale bar in (A) applies to B-D). Scale bar in (E) applies to (F-H). Scale bar in (I) applies to (J-L). Scale bar in (M) applies to (N-P). The scale bar in all images represents 40 μM.

History