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In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR - Fig 8

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posted on 2017-08-24, 18:04 authored by Faqing Huang, Joseph Rankin Spangler, Allen Yang Huang

Identification of positive colonies by colony PCR from the construction of (A) pIkB, (B) pDcEG, and (C) pDSADE. The expected correct DNA products are marked on the right sides of the gels. The smear bands below the correct DNA product bands are not consistent from different PCR reactions, but their identity and reasons for inconsistency are unknown.