ppat.1009030.s007.tif (414.72 kB)
In vitro phagocytosis assay using neutrophils from wild type (WT) and PGLYRP1-/- mice.
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posted on 2020-11-11, 18:34 authored by Akash Gupta, Gunjan Arora, Connor E. Rosen, Zachary Kloos, Yongguo Cao, Jiri Cerny, Andaleeb Sajid, Dieuwertje Hoornstra, Maryna Golovchenko, Natalie Rudenko, Ulrike Munderloh, Joppe W. Hovius, Carmen J. Booth, Christine Jacobs-Wagner, Noah W. Palm, Aaron M. Ring, Erol FikrigThe neutrophils isolated from mouse bone marrow were incubated with eFluor 670 dye-labeled B. burgdorferi at different ratios for 1 hour. The neutrophils were subsequently washed and analyzed by flow cytometer. (A) Histogram showing neutrophils that phagocytosed Borrelia. High and low represent the ratio of 30 and 1 (B. burgdorferi, 1x105). Y-axis represents relative cell counts calculated as percentage of the maximum events (Borrelia). (B) The graph show percent of phagocytic neutrophils plotted against Borrelia numbers. Results from three independent experiments are shown. The bars represent mean ± SEM and p-values were determined by Student t-test.
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exhibits borreliacidal activityPeptidoglycan Recognition Protein 1burgdorferi infectionspirochete Borrelia burgdorferiIFNburgdorferi sensu latosystems biology approachsecretome library screenDiverse host responses influenceCXCLyeast display screeningserum IgG levelsLyme borreliosis Lyme diseasePGLYRP 1yeast display library
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