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H. diminuta–infected IL-22-/- display delayed and/or prolonged expression of immunoregulatory factors.

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posted on 2016-04-07, 16:02 authored by José L. Reyes, Maria R. Fernando, Fernando Lopes, Gabriella Leung, Nicole L. Mancini, Chelsea E. Matisz, Arthur Wang, Derek M. McKay

Wild-type (WT) and IL-22-/- mice were infected with 5 H. diminuta and at time-points thereafter splenocytes (A) and mesenteric lymph node cells (MLN) (B) were excised and stimulated with conA (5 μg/ml) for 48 hr and IL-10 measured. (C) Shows increases in Foxp3 mRNA in the mid-jejunum of WT and IL-22-/- post-infection. (D) Flow cytometry revealed increased numbers of CD4+Foxp3+ splenocytes 8 days post-infection (dpi) in both WT and IL-22-/- mice (E, representative dot plots) (data are mean ± SEM; n = 6–9; * and # p<0.05 compared to strain control and time-matched WT, respectively).