GLI1 and PTCH1 expression in response to Hh pathway stimulation.

A) GLI1 and B) PTCH1 target gene induction. Each data point represents the expression fold change of stimulated cells from the same batch. Expression fold change was normalized to unstimulated values, which were set to 1. Two-tailed Student’s t-test was performed. P-values represented significant differences if they ranged from 0.01 to 0.05 (*), 0.001 to 0.01 (**), and <0.001 (***).