Drosophila wing size measurements per experimental batch 4–7.

PTEN variants were tested across 7 batches total; attp2 and PTEN-WT were repeated in each batch. (A-C) Adult wing size (in pixels) of PTEN variants assayed within each batch. Variants indicated as “GOF” had significantly smaller wings than PTEN-WT, “Like WT” were not significantly different from PTEN-WT, “Partial LOF” were significantly different from both PTEN-WT and attP2. “LOF” were significantly different from PTEN-WT and not significantly different from attP2. The wing size assay demonstrates the utility of Drosophila as a model system to test the relative function of PTEN variants. Each datum point in the scatter plot represents a single adult wing. Data are expressed as mean ± SD. Significant differences as stated were obtained from analysis within each batch using one-way ANOVA with post-hoc Tukey HSD.

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