Donson expression in the developing telencephalon.
(A-J) Darkfield micrographs show Donson expression in coronal (a.-d. and f.-j.) and sagittal (e.) sections of the telencephalon at the indicated developmental stages after in situ hybridization with a 35S-labeled probe. (K) The image demonstrates the signal intensity produced by the sense strand probe (sense) at P30. (L) Quantification of the Donson hybridization signal in the M1 region of the neocortex after subdividing into 10 equally-sized bins (bin 1 corresponds to the subpial layer). Graphs depict the positive area fraction as mean+SEM. (M) Donson hybridization signal in 10 neocortical bins (mean of the positive area fraction) expressed as fold change versus the signal of the sense probe. (N) The heat map shows a qPCR analysis of Donson expression levels in RNA extracts of dorsal telencephalon (E11.5 –E17.5) and cortex (P30). Values are given as percentage of the overall mean. (O) The confocal image shows fluorescence signals in the E12.5 neocortex for reporter mice expressing nuclear GFP and cell membrane tdT from the Tbr2 locus. Stable nuclear GFP labels IPC and IPC-derived neurons [22]. The tdT signal emerges later than GFP and labels IPC-derived neurons [22]. The scatter plot demonstrates FACS from E12.5 dorsal telencephalon. Four cell populations were defined using the GFP/ tdT signals of the Tbr2 reporter. The heat maps show qPCR analysis of Pax6, Tbr2, and Donson in the four populations. Values were first normalized to Gapdh and then expressed as percentage of the overall mean of the heat map. Measurements and statistics are summarized in S1A Table. Abbreviations: CA1, hippocampal subfield CA1; cc, corpus callosum; CP, cortical plate; Ctx, cerebral cortex; DG, dentate gyrus; IZ, intermediate zone; MZ, marginal zone; OE, olfactory epithelium; PP, preplate, SVZ, subventricular zone, VZ, ventricular zone. Scale bars: 300 μm (a. and c.), 200 μm (g.), 20 μm (o.).
