pone.0251080.g003.tif (335.79 kB)
CD8 expression by qPCR correlates with PD-L1 expression by IHC for 50% cut-off.
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posted on 2021-05-06, 17:30 authored by Akram Alwithenani, Drew Bethune, Mathieu Castonguay, Arik Drucker, Gordon Flowerdew, Marika Forsythe, Daniel French, John Fris, Wenda Greer, Harry Henteleff, Mary MacNeil, Paola Marignani, Wojciech Morzycki, Madelaine Plourde, Stephanie Snow, Paola Marcato, Zhaolin XuA total of 49 fresh lung tumors were evaluated for PD-L1 expression by IHC and quantified for CD8, CD3 and CD45 mRNA by RT-qPCR. (A) CD45 (RT-qPCR) did not correlate with PD-L1 (IHC, 1% and 50% cut-off). (B) Also, CD3 marker (qPCR) was not significantly correlated with PD-L1 (IHC, 1% and 50% cut-off). (C) CD8 marker (RT-qPCR) was significantly correlated with PD-L1 (IHC) for 50% cut-off but not for 1% cut-off.
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non-small cell lung cancer casesProfiling non-small cell lung cancerPD-L 1 expressionKRAStarget driver gene mutationsPD-L 1 correlatesunsorted tumor samplesROSPD-L 1 levelsdriver gene mutationstumor cellsPD-L 1 mRNA expressionlung cancer patientslung cancer subtypesPD-L 1 mRNA levelsALKEGFRPD-L 1 protein levelPD-L 1 immunotherapiesprotein expressionBRAFPIK 3CA mutations
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