B. burgdorferi specific IgM titers in mice.

Antibody levels in uninfected wild type BALB/c (WT) and PGLYRP1^-/- mice were compared with those in the infected ones (at least n = 7 in each group). Results from two independent experiment are shown. Whole-cell lysate of B. burgdorferi was coated on a microtiter plate and serum from either uninfected WT, infected WT, uninfected PGLYRP1^-/- or infected PGLYRP1^-/- mice was used at 1:200 dilution. The binding was measured by the secondary Goat anti-mouse IgM HRP-conjugated antibody. No significant difference in IgM level was observed in infected WT compared to infected PGLYRP1 knockout mice. Each data point represents an individual animal in the corresponding group. The bars represent mean ± SEM and p-values determined by student t-test.

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