posted on 2024-02-03, 00:48authored byJ. Hunter Cox, Richard McCain Jr., Tran, Shoba Swaminathan, Holland Smith, Gerardo Piroli, Michael Shtutman, michael walla, william cotham, Norma FrizzellNorma Frizzell
Itaconate and fumarate have roles as immunometabolites modulating the macrophage response to inflammation. Both immunometabolites chemically modify protein cysteine residues to modulate the immune response. Itaconate and fumarate levels change dynamically, whereas their stable protein modifications can be quantified by mass spectrometry. This method distinguishes itaconate and fumarate-derived protein modifications and will allow researchers to quantify their contributions in isolated cell types and tissues across a range of metabolic diseases.
Funding
This work was supported by the National Institutes of Health R01NS126851, R56NS116174, R01NS092938 and R21058586, the National Science Foundation 1828059 and an ASPIRE II grant from the University of South Carolina.