Zip14^−/− mice exhibit normal iron absorption but altered iron homeostasis.

(A) WT and Zip14^−/− mice were administered LPS (2 mg/kg or saline 0.5 mL; ip), 18 hr before being killed. (A) Serum and liver iron concentrations were measured by AAS. Liver non-heme-iron was measured colorimetrically. (B) Fasted WT and Zip14^−/− mice received 2 µCi of ⁵⁹Fe by gavage and were killed 24 hr later. Percent absorption was calculated from the radioactivity administered. Serum and liver iron uptake was calculated from the specific activity of the ⁵⁹Fe. (C) Transcript abundance for liver hepcidin, TfR-1, DMT1 and ferritin was measured by qPCR and expressed relative to TBP mRNA as the normalizer. Values are mean ± SE, n = 5−10.