YS110 and CD26 Translocate to the Nucleus In Vivo.
H&E staining (A and F) and fluorescence analysis (B–E and G–J) of malignant mesothelioma tumors in NOG mice inoculated with JMN cells were shown. These tumors were removed 6 hours after one intratumoral injection (1 µg/a tumor, volume is 100 µL) of Alexa647-human IgG1 (A–E) or Alexa647-YS110 (F–J). In the overlaid image, CD26 expression is indicated in red (B, D, G, and I), Alexa647-labeled antibodies are shown in green (C, D, H and I), and the nucleus is shown in blue (D and I). Colocalization of CD26 and YS110 in the nucleus appears as white (I, arrows). This localization of Alexa647-labeled antibody (green) in the nucleus (red) is confirmed as yellow (arrows) (J). Similar results were obtained with three different mice. Scale bars, 20 µm (A and F) and 10 µm (B–E and G–J).