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Viral IE86, like cellular MCM3, causes relocalisation of MCM3AP to the nucleus.

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posted on 2013-02-19, 21:28 authored by Emma Poole, Mark Bain, Linda Teague, Yoshinori Takei, Ron Laskey, John Sinclair

293-T cells were co-transfected with GFP-tagged MCM3AP (A and B) together with MCM3 or pCDNA3 (A); pCDNA3-IE72 (IE72) or pCDNA3-IE86 (IE86) (B). After fixing, MCM3AP-GFP expressing cells were analysed by co-staining for expression of MCM3, IE86 (IE) or IE72 (IE) as indicated. DNA (Hoechst 33342) staining is also shown.