Using live cell impedance based platforms to monitor cell behaviour.

xCELLigence (ACEA) platforms were used to monitor cells cultured under the same conditions as those performed in the MTT, WST and LDH assays. All experiments were carried out using E-plates following the ACEA manual protocol where N = 3. (A) Shows MCF-7 cells seeded at 27,000 cells, with and without the presence of WDW (with appropriate controls and blanks). The data indicates that there is an increase in the cell index number a measure of increased impedance across the surface of the plate. (C) Shows L929 seeded at 10,000 cells with and without the presence of WDW (with appropriate controls and blanks). The data indicates that there is an increase in the cell index number a measure of increased impedance across the surface of the plate. (B), (D). MCF-7 cells and L929 cells grown on separate E plates together with increasing concentrations of WDW's to show how the Germanium nanowires themselves have no effect on the impedance as measured by the electrodes.