Typical 2d reconstructions showing x-ray attenuation with depth in the lesions at different remineralization times.

a) control lesion (0 time) showing protected portion at the left, the severely demineralized region which gradually blends with normal dentin. Widened dentin tubules were apparent at the depth of the lesion. b) 7 day remineralization treatment seems more complete in the deeper part of the lesion. c) 14 day remineralization shows some unevenness in the remineralization, but much of the lesion appeared fully mineralized. d) 28 day remineralization was similar to 14 day specimens. Note there is no apparent shrinkage in any of the specimens as the imaging was done in fully hydrated tissue (see supplemental Figure 1). Dotted line shows approximate lesion depth, magnification bar = 100 µm. A movie at 10X, demonstrating widened tubules at the lesion site is also provided as supplemental information.